The research studies concern the microbe proteomic issues related to the animal health (including animal products), the food safety and public health. More specifically, the focus of the lab is on the mechanisms of proteomic regulation induced by antibiotic resistance, the study of proteins secreted by lactic bacteria to enhance food safety, metaproteomics aimed at studying microbial groups and microbiome in food. The lab also developed interesting research material related to the fertility of productive livestock. The proteomic laboratory has been an important reference point within the European COST- FARM ANIMAL PROTEOMICS project (2010-2014); is currently involved in the European COST- IMPARAS project (a study of the protein allergens derived from insects). It is also an operative unit within the European project, PROLIFIC (2013-2017) (FP7-KBBE) which conducts proteomic studies applied to reproduction in bovine submitted to alternative diets. The proteomic laboratory collaborates with the department of Veterinary Sciences and Public Health of the University of Milan.
Techniques applied: one- and two-dimensional electrophoresis, also on peptides; extraction from complex matrixes (bead beating, colons, sonication, homogenisation) comassie blu dying, silver and fluorescence (2D-DIGE) staining, immunoblotting for the qualification of proteomic, colorimetric and chemiluminescent data, mass spectrometry (MALDI-Tof e LC/MS-MS) (this in collaboration); immune-proteomics; bioinformatics: image analysis (progenesis samespots), metaproteomic analysis (megan, unipept, metaproteome analyzer, blast) and interatomic analysis (string, cytoscape, ingenuity).
“Hohenheimer SchlossGeister” – Stress & Aging